1. What factors are involved in the separation of the pigments?

2. Would you expect the R_f value of a pigment to be the same if a different solvent were used? Explain.

3. What type of chlorophyll does the reaction center contain? What are the roles of the other pigments?

4. What is the function of DPIP in this experiment?

5. What is the source of electrons that will reduce DPIP?

6. What was measured with the spectrophotometer?

7. What is the predicted effect of darkness on the reduction of DPIP? Explain.

8. What is the effect of boiling the chloroplasts on the subsequent reduction of DPIP? Explain.

9. What reasons can you give for the difference in the percentage of transmittance between the live chloroplasts that were incubated in the light and those that were kept in the dark?