Enzyme Catalysis Lab Questions

1. Determine the initial rate and the rates between each of the time points and Record the results.

Time Intervals (s)

  Initial 0 to 10 10 to 30 30 to 60 60 to 120 120 to 180 180 to 360
Rate            

2. When was the rate the highest? Explain why.

3. When was the rate the lowest? Explain why.

4. Explain the inhibiting effect of sulfuric acid on the function of catalase. Relate this to enzyme structure and chemistry.

5. Predict the effect that lowering the temperature would have on the rate of enzyme activity. Explain your prediction.

6. Design a controlled experiment to test the effect of varying pH, temperature, or enzyme concentration.